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KMID : 0387719960070010027
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Korean Journal of Blood Transfusion
1996 Volume.7 No. 1 p.27 ~ p.32
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Frozen-Thawed RBCs transfusion in the Dog
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임채승
김병수/정현진/김영기/이갑노/김준석/한규섭
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Abstract
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The cryopreservation of Red Blood Cells has many advantages of which the most important one is that it can be stored for a long period. However, in Korea, Research regarding frozen blood is still in its early stage . We evaluated the effects of
transfusion of the frozen-thawed RBCs in dogs. The whole bloods were collected from 5 dogs, and the packed RBCs were obtained by centrifugation method. We made the frozen RBCs by using 40% glycerol method and stored it in -80℃ refrigerate for 1
month.
The frozen RBCs were thawed in the 37℃ water bath and washed by Cell washer according to the standard protocol. And evaluated the status of them being compared with that of the unfrozen The majority of the results were satisfactory to the
allowable
limit except high plasma hemoglobin and potassium. The frozen-thawed bloods were transfused to the two dogs and carefully observed the effects and its complications. The results were that the average value of the hemoglobin was elevated about
0.6g/dL
more after transfusion than before. And there were no significant complication related to the transfusion. Thus, The frozen thawed blood transfusions in case of the experiment with dogs were proved to be safe and as effective as fresh blood, and
The
above method appeared to be feasible to human blood.
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KEYWORD
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